SubtiBank SubtiBank
Version comparison:

2019-03-05 10:15:042025-05-10 10:05:52

description

threonine synthase

threonine synthase, has a minor threonine dehydratase ([[protein|IlvA]]) activity

locus

BSU32250

BSU_32250

outlinks

bsu

BSU32250

BSU_32250

The protein

Protein family

threonine synthase family (according to UniProt)

threonine synthase family (single member, according to UniProt)

The protein

Structure

[PDB|6CGQ]

[PDB|6CGQ] (complexed with PLP and PLP-Ala) [pubmed|30830751]

[PDB|6NMX] (complexed with PLP and (Z)-L-2-amino-5-phosphono-3-pentenoic acid (APPA)) [Pubmed|30830751]

Biological materials

Mutant

GP3030 Δ''[[gene|thrC]]''::''spec'', available in [SW|Jörg Stülke]'s lab

1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab

BKE32250 ([[gene|thrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

BKK32250 ([[gene|thrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

GP3030 Δ[[gene|thrC]]::''spc'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab

BKE32250 ([[gene|thrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

BKK32250 ([[gene|thrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

References

12107147, 3098560, 24163341, 15378759, 27260660, 30830751

12107147, 3098560, 24163341, 15378759, 27260660, 30830751, 4630852, 4199513, 32743959

Gene

Phenotypes of a mutant

auxotrophic for threonine [pubmed|32743959]