2019-03-05 10:15:042025-05-10 10:05:52
description
threonine synthase
threonine synthase, has a minor threonine dehydratase ([[protein|IlvA]]) activity
locus
BSU32250
BSU_32250
outlinks
bsu
BSU32250
BSU_32250
The protein
Protein family
threonine synthase family (according to UniProt)
threonine synthase family (single member, according to UniProt)
The protein
Structure
[PDB|6CGQ]
[PDB|6CGQ] (complexed with PLP and PLP-Ala) [pubmed|30830751]
[PDB|6NMX] (complexed with PLP and (Z)-L-2-amino-5-phosphono-3-pentenoic acid (APPA)) [Pubmed|30830751]
Biological materials
Mutant
GP3030 Δ''[[gene|thrC]]''::''spec'', available in [SW|Jörg Stülke]'s lab
1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab
BKE32250 ([[gene|thrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
BKK32250 ([[gene|thrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
GP3030 Δ[[gene|thrC]]::''spc'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab
BKE32250 ([[gene|thrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
BKK32250 ([[gene|thrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
References
Gene
Phenotypes of a mutant
auxotrophic for threonine [pubmed|32743959]